Prevention by the CXCR2 antagonist SCH527123 of the calcification of porcine heart valve cusps implanted subcutaneously in rats

Introduction Calcification is a main cause of bioprosthetic heart valves failure. It may be promoted by the inflammation developed in glutaraldehyde (GA)-fixed cusps bioprosthesis. We tested hypothesis that antagonizing C-X-C chemokines receptor 2 (CXCR2) prevent calcification GA-fixed porcine aortic valves. Materiel and methods Four-week-old Sprague Dawley males were transplanted with valve isolated from independent pigs implanted into dorsal wall. Four groups 6 rats compared: GA-free or treated 1 mg/kg/day SCH5217123 (a CXCR2 antagonist) intraperitoneally (IP) subcutaneously (SC) around xenograft, for 14 days. Then, underwent blood count before xenografts have been explanted histology biochemistry analyses. Results A strong was induced GA pre-incubation. However, we observed significant decrease this effect SCH527123 IP SC. Implantation associated increase white cell count, an significantly prevented SCH527123. In addition, expression CD3, CD68 markers reduced as compared to those non-treated rats. Conclusion The This partly result inhibition GA-induced infiltration T-cells macrophages xenograft.